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See more. In enzymology, DNA-(apurinic or apyrimidinic site) lyase, also referred to as DNA-(apurinic or apyrimidinic site) 5'-phosphomonoester-lyase (systematic name) or DNA AP lyase (EC 4.2.99.18) is a class of enzyme that catalyzes the chemical reaction of the cleavage of the C 3'-O-P bond 3' from the apurinic or apyrimidinic site in DNA via beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate. In some cases, an AP lyase activity of the enzyme desaturates the AP sugar and cleaves its 3′-phosphate link. However, in all cases, an AP endonuclease then breaks the phosphate bond attached to the 5′-C of the AP sugar to leave an upstream 3′-OH end suitable for extension by a repair polymerase. AP Endonucleases.

Such studies were designed to determine if Ntg1p and Ntg2p prefer speci®c bases located opposite abasic sites and whether these lesions are processed with a catalytic ef®ciency similar to Apn1p, the major hydrolytic AP endo-nuclease of yeast. Proteintech Anti-ATP citrate lyase Polyclonal, Catalog # 15421-1-AP. Tested in Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (Flow) and Immunoprecipitation (IP) applications. This antibody reacts with Human, Mouse, Rat samples.

and immature ovariectomised and immature and intact Alpk:AP rats. phenylalanine ammonia-lyase (PAL), which converts phenylalanine to BER: "base excision repair"; den felaktiga basen tas bort av ett DNA-glykosylas, nucleas/helikas, ett AP-site skapas.

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8-oxo-dG glycosylase/AP lyase activity in crude mitochondrial extracts was measured by an oligonucleotide incision assay, as previously described . Briefly, RHM or RLM were resuspended in a buffer containing 20 mM HEPES-KOH (pH 7.6), 1 mM EDTA, 2 mM DTT, 300 mM KCl, 5% glycerol and 0.05% Triton X-100.

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Generates 3'-phosphor-alpha,beta-unsaturated aldehyde (3'-PUA) as a primary 5-meC excision intermediate (PubMed: 25228464 ). We previously demonstrated mammalian Ku has intrinsic 5' deoxyribosephosphate (5'dRP) and apurinic/apyrimidinic (AP) lyase activity, and showed this activity is important for excising abasic site damage from ends.

Allergena proteiner i pollen kan Savvatianos S, Konstantinopoulos AP, Borgå Å, Stavroulakis G, Lidholm J, Borres MP,. Manousakis E compromised in vivo by the metabolites accumulating in 3-hydroxy-3-methylglutaryl-CoA lyase deficiency in A. P. Lin Brain imaging and behavior.2012, Vol. av S Johansson — literature study different organisms and substances that have the possibility to be ap- proved for organic potato med enzymet alliin-lyase.
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A key feature of biological sources of strand breaks is associated nucleotide damage, including base loss (abasic or apurinic/apyrimidinic (AP) sites). At single-strand breaks, 5'-terminal abasic sites are excised by the 5'-deoxyribose-5-phosphate (5'-dRP) lyase activity Kevin G. Pinz, Daniel F. Bogenhagen By virtue of its binding to AP sites, PARP-1 could be poised for its role in base excision repair, pending DNA strand incision by APE1 or the 5′-dRP/AP lyase activity in PARP-1.

(redirected from AP lyase) APEX1 A gene on chromosome 14q11.2 that encodes a major apurinic/apyrimidinic (AP) endoDNAase, which is involved in the DNA base excision repair (BER) pathway of DNA lesions induced by oxidative and alkylating agents, and in the redox regulation of transcriptional factors. We previously demonstrated mammalian Ku has intrinsic 5' deoxyribosephosphate (5'dRP) and apurinic/apyrimidinic (AP) lyase activity, and showed this activity is important for excising abasic site damage from ends. Here we employ systematic mutagenesis to clarify the protein requirements for this activity. During AP site cleavage by the AP lyase activity of DNA glycosylases, an aldimine intermediate between the C1′ atom of deoxyribose and a primary amine group in the protein is formed Bifunctional DNA glycosylase/lyase, which excises 5-methylcytosine (5-meC) and 5-hydroxymethylcytosine (5-hmeC), leaving an apyrimidinic (AP) site that is subsequently incised by the lyase activity (PubMed: 25240767 ).
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ap endonuclease, ref-1, ape1/ref-1, apex1, ape/ref-1, apurinic/apyrimidinic endonuclease 1, ap lyase, ape-1, endo iii, ap-endonuclease, more top print hide show all columns Go to Synonym Search Please wait a moment until the data is sorted.